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The 2 protein bands from forty kDa to 30 kDa have been absent immediately after 24 h incubation at forty five °C. For the rest of the bands, a variety of concentrations of all 3 proteases retained protein bands corresponding to their primary molar masses (Determine 6a,b). The presence of bands at reduced molecular body weight in fish roe ahead of hydrolysis was surprising. Within the report by Ghelichi et al. [eight], minimal peaks of a relatively small molecular body weight selection (3–fourteen kDa) have been observed in defatted carp roe homogenate, most likely as a consequence of an autolytic system taking place in fish roe and/or even the defatting process resulting in the formation of very low-molecular-bodyweight peptides.

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The improved amenability of proteins together with other hydrolysable components from the roe homogenate to hydrolysis following defatting could reveal this phenomenon. The hydrophilicity with the a few enzymes might be a contributing factor.

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